Search results for " Cell-penetrating peptide"

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Transportan 10 Induces Perturbation and Pores Formation in Giant Plasma Membrane Vesicles Derived from Cancer Liver Cells

2023

Continuous progress has been made in the development of new molecules for therapeutic purposes. This is driven by the need to address several challenges such as molecular instability and biocompatibility, difficulties in crossing the plasma membrane, and the development of host resistance. In this context, cell-penetrating peptides (CPPs) constitute a promising tool for the development of new therapies due to their intrinsic ability to deliver therapeutic molecules to cells and tissues. These short peptides have gained increasing attention for applications in drug delivery as well as for their antimicrobial and anticancer activity but the general rules regulating the events involved in cell…

cell-penetrating peptidesdi-4-ANEPPDHQprotein–membrane interactionsgiant plasma membrane vesiclesprotein–membrane interactions; cell-penetrating peptides; Transportan 10; giant plasma membrane vesicles; phasor approach; Nile Red; di-4-ANEPPDHQ; membrane hydrationTransportan 10Nile RedMolecular BiologyBiochemistryphasor approachSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)membrane hydration
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Increased carrier peptide stability through ph adjustment improves insulin and pth(1-34) delivery in vitro and in vivo rather than by enforced carrie…

2020

Oral delivery of therapeutic peptides is hampered by their large molecular size and labile nature, thus limiting their permeation across the intestinal epithelium. Promising approaches to overcome the latter include co-administration with carrier peptides. In this study, the cell-penetrating peptide penetratin was employed to investigate effects of co-administration with insulin and the pharmacologically active part of parathyroid hormone (PTH(1-34)) at pH 5, 6.5, and 7.4 with respect to complexation, enzymatic stability, and transepithelial permeation of the therapeutic peptide in vitro and in vivo. Complex formation between insulin or PTH(1-34) and penetratin was pH-dependent. Micron-size…

insulinmedicine.medical_treatmentlcsh:RS1-441Pharmaceutical ScienceParathyroid hormonePeptide02 engineering and technologyArticlelcsh:Pharmacy and materia medica03 medical and health sciencespenetratinIn vivoCarrier peptide Cell-penetrating peptide Insulin Intestinal peptide delivery Penetratin PTH(1-34)Membrane activitymedicine030304 developmental biologychemistry.chemical_classification0303 health sciencesLiposomecarrier peptideChemistryInsulinPermeationintestinal peptide delivery021001 nanoscience & nanotechnologyBiophysicsCell-penetrating peptide0210 nano-technologyPTH(1-34)cell-penetrating peptide
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